Research

Bio-Imaging & Optical Systems (BIOS) Research Lab

BIOS research lab

Lab head: Prof. Manish Kumar

Thrust areas in the BIOS research lab*:

1. Optical microscopy: Light-sheet, fluorescence, confocal etc.
2. Optical imaging: Schlieren, photomicrography etc.
3. Optical systems: Seismometer, meteorology etc.

*These are only indicative research areas. We are open to expanding our research area.

 

Fig 1: Compact light-sheet microscopy system. (a) 3D rendering; (b) schematics with typical control signals; and (c) a customised water-immersion flexible chamber.

 

Fig 2: 3D imaging results in a GFP expressing mouse brain slice. Neurons and finer processes are visible here. Scale bar: 100 µm.

 

Come join us: We at the BIOS research laboratory appreciate and welcome creative thinkers. An ideal BIOS lab member loves optics and solves real problems. He/she/they believe in interdisciplinary research scope, enjoy tinkering, and get involved in both hands-on experiments & programming. We will work together to discuss problems, generate innovative ideas, shape our ideas into real-life instruments, and disseminate it to the research community and society. Feel free to write to Manish (kmanish@iitd.ac.in).

 

Recent publications:

1. Y. Kozorovitskiy and M. Kumar, "Scanned oblique plane illumination microscopy”, US Patent 10802255 (2020)
2. M. Kumar, S. Kishore, D. McLean and Y. Kozorovitskiy, "Crossbill: an open access single objective light-sheet microscopy platform", bioRxiv (2021) doi: 10.1101/2021.04.30.442190
3. A. A. Shemetov, ….. M. Kumar et.al, "A near-infrared genetically encoded calcium indicator for in vivo imaging", Nature Biotechnology 39, 368–377 (2021)
4. M. Kumar, and Y. Kozorovitskiy, "Tilt (in)variant lateral scan in oblique plane microscopy: a geometrical optics approach", Biomedical Optics Express 11 (6), 3346-3359 (2020)
5. M. Kumar, and Y. Kozorovitskiy, "Tilt-invariant scanned oblique plane illumination microscopy for large-scale volumetric imaging", Optics Letters 44 (7), 1706-1709 (2019)
6. M. Kumar, S. Kishore, J. Nasenbeny, D. McLean and Y. Kozorovitskiy, "Integrated one- and two-photon scanned oblique plane illumination (SOPi) microscopy for rapid volumetric imaging", Optics Express 26 (10), 13027-13041 (2018)